Bioinformatics(1) •
Exercise(2) •
Extraction(15) •
Library Prep(24) •
Methylation(24) •
PCR(9) •
Processing(39) •
Protocol(14)
Bioinformatics 1
Exercise 2
- Forking the Putnam Open Lab Notebook for Your GitHub • September 26, 2018
- Pipetting Exercises for Starting Labwork • February 6, 2019
Extraction 15
- 2nd Qiagen Genomic Tip HMW DNA Extraction of Pocillopora meandrina • February 1, 2021
- 2nd Qiagen Genomic Tip HMW DNA Extraction of Porites lobata • February 5, 2021
- 2nd Try Porites astrodies RNA Extraction with Purelink Kit • November 20, 2020
- 2nd Try Qiagen Genomic Tip HMW DNA Extraction of Fire Coral • April 20, 2021
- 3rd Try Porites astrodies RNA Extraction with Purelink Kit • November 25, 2020
- CTAB Extraction of Pocillopora meandrina • January 22, 2021
- Porites astrodies RNA Extraction with Purelink Kit • November 16, 2020
- Porites astrodies RNA Extraction with Zymo Kit • December 8, 2020
- Qiagen Genomic Tip HMW DNA Extraction of Fire Coral • April 12, 2021
- Qiagen Genomic Tip HMW DNA Extraction of Pocillopora acuta • February 12, 2021
- Qiagen Genomic Tip HMW DNA Extraction of Pocillopora meandrina • November 10, 2020
- Qiagen Genomic Tip HMW DNA Extraction of Porites lobata • December 3, 2020
- Sampling, Extraction, and mtORF Amplification and Sanger Sequencing Prep of 2 Aquarium Corals • January 8, 2021
- Second Qiagen Genomic Tip for HMW DNA Extraction of Porites asteroides • March 12, 2020
- Using the Qiagen Genomic Tip for HMW DNA Extraction of Porites asteroides • March 6, 2020
Library Prep 24
- 1 Final WGBS of K-Bay Bleaching Samples • June 3, 2021
- 16 WGBS of K-Bay Bleaching Samples • May 21, 2021
- 2nd 12 WGBS of K-Bay Bleaching Samples • May 14, 2021
- 2nd Try TagSeq Library Prep Test • July 7, 2021
- 3rd Try TagSeq Library Prep Test • August 6, 2021
- 4th Try TagSeq Library Prep Test • August 9, 2021
- Dilute, Re-Qunatify, and Re-Tape 1st KBay WGBS • May 13, 2021
- EpiRAD ddRAD Mo'orea Pocillopora Library Prep and Protocol • July 1, 2019
- Final 2 Re-do WGBS Library Prep for Danielle's Pocillopora Samples • October 13, 2020
- First 12 WGBS of K-Bay Bleaching Samples • May 10, 2021
- First 16 WGBS Library Prep for Danielle's Pocillopora Samples • September 24, 2020
- MBD Enriched Oyster DNA Pico Methyl-Seq Library Prep • January 9, 2020
- Methylation Comparison WGBS With Pico Methyl Seq Library Prep Kit First Try • September 19, 2019
- Methylation Comparison WGBS and MBDBS With Pico Methyl Seq Library Prep Kit • September 26, 2019
- Oyster Nutrient Enrichment Treatment RNA Lib Prep with Zymo-Seq RiboFree Total RNA Library Prep Kit • October 18, 2019
- Pico Methyl Seq Library Prep Kit Test • August 13, 2019
- RRBS Library Prep of Samples for Methylation Comparison • October 24, 2019
- Reconditioning PCR of KBay WGBS Libraries • May 28, 2021
- Second 16 and 1 Redo WGBS Library Prep for Danielle's Pocillopora Samples • October 2, 2020
- TagSeq Library Prep Test • June 21, 2021
- Troubleshoot and Redo Some Samples for Methylation Comparison With Pico Methyl Seq Library Prep Kit • October 8, 2019
- Zymo Pico Methyl Seq Library Prep for WGBS Protocol • September 18, 2020
- Zymo-Seq RiboFree Total RNA Library Prep Kit Soft RNA • September 9, 2019
- Zymo-Seq RiboFree Total RNA Library Prep Kit Test • September 5, 2019
Methylation 24
- 1 Final WGBS of K-Bay Bleaching Samples • June 3, 2021
- 10 Minute Sonication MethylMiner Test • August 7, 2019
- 16 WGBS of K-Bay Bleaching Samples • May 21, 2021
- 2nd 12 WGBS of K-Bay Bleaching Samples • May 14, 2021
- Bisulfite Conversion Test and TapeStation • August 12, 2019
- Dilute, Re-Qunatify, and Re-Tape 1st KBay WGBS • May 13, 2021
- EpiRAD ddRAD Mo'orea Pocillopora Library Prep and Protocol • July 1, 2019
- Final 2 Re-do WGBS Library Prep for Danielle's Pocillopora Samples • October 13, 2020
- First 12 WGBS of K-Bay Bleaching Samples • May 10, 2021
- First 16 WGBS Library Prep for Danielle's Pocillopora Samples • September 24, 2020
- Invitrogen MethylMiner Methylated DNA Enrichment Kit Test • August 1, 2019
- MBD Enriched Oyster DNA Pico Methyl-Seq Library Prep • January 9, 2020
- MBD of Samples for Methylation Comparison • September 23, 2019
- Methylation Comparison WGBS With Pico Methyl Seq Library Prep Kit First Try • September 19, 2019
- Methylation Comparison WGBS and MBDBS With Pico Methyl Seq Library Prep Kit • September 26, 2019
- Pico Methyl Seq Library Prep Kit Test • August 13, 2019
- RRBS Library Prep of Samples for Methylation Comparison • October 24, 2019
- Reconditioning PCR of KBay WGBS Libraries • May 28, 2021
- Restriction Enzyme Digest Test for Mo'orea Porites and Pocillopora ddRAD and EpiRAD • May 2, 2019
- Second 16 and 1 Redo WGBS Library Prep for Danielle's Pocillopora Samples • October 2, 2020
- Simulating Double Digest to get Fragment Distributions • May 5, 2019
- Testing Sonication for MBD • July 30, 2019
- Troubleshoot and Redo Some Samples for Methylation Comparison With Pico Methyl Seq Library Prep Kit • October 8, 2019
- Zymo Pico Methyl Seq Library Prep for WGBS Protocol • September 18, 2020
PCR 9
- Clean-up, Quantification, and Prep for mtORF Test Sequence Samples • July 31, 2020
- Mo'orea Connect Pocillopora mtORF Amplification and Sanger Sequencing Prep Processing • September 3, 2020
- PocHistone Amplification and Sanger Sequencing Prep Protocol • January 25, 2021
- Pocillopora Samples from Danielle mtORF Amplification and Sanger Sequencing Prep Processing • August 27, 2020
- Primer Resuspention and Test PCR for mtORF Pocillopra Species Determination • July 25, 2020
- Sampling, Extraction, and mtORF Amplification and Sanger Sequencing Prep of 2 Aquarium Corals • January 8, 2021
- Second Test PCR for mtORF Pocillopra Species Determination • July 27, 2020
- mtORF Amplification and Sanger Sequencing Prep Protocol • August 27, 2020
- mtORF Amplification of HMW P. acuta • February 22, 2021
Processing 39
- 2nd Qiagen Genomic Tip HMW DNA Extraction of Pocillopora meandrina • February 1, 2021
- 8 Mo'orea Coral Sample Extractions • April 10, 2019
- Acropora hyacinthus Larvae Test DNA/RNA Extraction • February 24, 2021
- Airbrushing Coral for Bacterial Composition Analysis with Homogenate Concentration and Test DNA Extraction • February 5, 2019
- CTAB Extraction of Pocillopora meandrina • January 22, 2021
- Continuing Through-put Mo'orea Coral Extractions • April 18, 2019
- Continuing Through-put Mo'orea Coral Extractions Five • May 1, 2019
- Continuing Through-put Mo'orea Coral Extractions Four • April 29, 2019
- Continuing Through-put Mo'orea Coral Extractions Three • April 24, 2019
- Continuing Through-put Mo'orea Coral Extractions Two • April 22, 2019
- EpiRAD ddRAD Mo'orea Pocillopora Library Prep and Protocol • July 1, 2019
- Half Volume Montipora Larvae Test DNA/RNA Extraction • March 8, 2021
- Invitrogen MethylMiner Methylated DNA Enrichment Kit Test • August 1, 2019
- MBD of Samples for Methylation Comparison • September 23, 2019
- MethylFlash Global DNA Methylation ELISA on Mcap Bleach/Non-Bleach from HI • April 2, 2021
- Mo'orea Connect Pocillopora mtORF Amplification and Sanger Sequencing Prep Processing • September 3, 2020
- Mo'orea Test Extractions Part 3 • March 28, 2019
- Montipora Eggs and Bundles Troubleshooting and Mo'orea Test DNA/RNA Extraction • March 17, 2019
- Montipora Larvae DNA/RNA Extraction 1 • March 11, 2021
- Montipora Larvae DNA/RNA Extraction 2 • March 17, 2021
- Montipora Larvae DNA/RNA Extraction 3 • March 22, 2021
- Montipora Larvae DNA/RNA Extraction 4 • March 23, 2021
- Montipora Larvae DNA/RNA Extraction 5 • March 29, 2021
- Montipora Larvae DNA/RNA Extraction 6 • April 5, 2021
- Montipora Larvae DNA/RNA Extraction 7 • April 15, 2021
- Montipora Larvae DNA/RNA Extraction 8 • April 21, 2021
- Montipora Larvae Test DNA/RNA Extraction 2021 • March 2, 2021
- Montipora capitata Sample M1312 for Genome Prep • January 19, 2021
- Pocillopora Samples from Danielle mtORF Amplification and Sanger Sequencing Prep Processing • August 27, 2020
- Porites astreoides RNA/DNA Extractions for RNA Seq Test • August 19, 2019
- Qiagen Genomic Tip HMW DNA Extraction of Pocillopora meandrina • November 10, 2020
- Quality Check of RNA Samples From Biomineralization Project • February 10, 2021
- Re-Tapestation of Porties lobata HMW DNA • January 27, 2021
- Sampling, Extraction, and mtORF Amplification and Sanger Sequencing Prep of 2 Aquarium Corals • January 8, 2021
- Starting Through-put Mo'orea Coral Extractions • April 15, 2019
- Testing Extraction of More Pocillopora and Porites Mo'orea Samples • March 20, 2019
- Testing Quick-DNA Kit on Mo'orea Corals and Tide Pool Mytilus • April 4, 2019
- Tide pool Flux Mytilus Test DNA RNA Extraction • December 19, 2018
- mtORF Amplification of HMW P. acuta • February 22, 2021
Protocol 14
- DNA/RNA Extraction Protocol for Coral Larvae • March 12, 2021
- Forking the Putnam Open Lab Notebook for Your GitHub • September 26, 2018
- General Template For Sample Processing Notebook Post • March 26, 2020
- General Template For Writing A Protocol in Your Lab Notebook • March 25, 2020
- PPP Lab Agarose Gel Protocol • November 27, 2018
- PPP Lab Genomic and D5000 Tape Station Protocol • July 30, 2019
- PPP Lab Qsonica Protocol • July 31, 2019
- PPP Lab RNA Tape Station Protocol • March 7, 2019
- PocHistone Amplification and Sanger Sequencing Prep Protocol • January 25, 2021
- Qiagen Genomic Tip HMW DNA Extraction Protocol • April 18, 2021
- Workflow for Uploading Raw Sequences to NCBI SRA • April 2, 2020
- Zymo Pico Methyl Seq Library Prep for WGBS Protocol • September 18, 2020
- dsDNA and RNA Qubit Quantification Protocol • March 8, 2019
- mtORF Amplification and Sanger Sequencing Prep Protocol • August 27, 2020