Block 10 EecSeq Probe Synthesis, Hybridization, and Capture
Synthesis of EecSeq Probes from Block 10 cDNA Libraries and Hybridization of Probes to DNA Libraries Normalization Pooling of cDNA libraries, 200ng each Sample μl cDNA B10T24J1 6.21 B10T24J2 6.29...
Block 10 DNA Library Prep
DNA Library Prep for CASE EecSeq Block 10 Using the KAPA HyperPrep DNA Library Prep Kit on 18 DNA samples in 10mM Tris HCl pH 8 and 500ng from block...
Block 10 Stranded mRNA Library Prep
Stranded mRNA Library Prep for CASE EecSeq Block 10 Following half reactions from the KAPA Biosystems Stranded mRNA-Seq Kit, also see the EeqSeq protocol Capture and Fragmentation Calculated number of...
CASE Block 12 Probe Synthesis and Hybridization
Synthesis of EecSeq Probes from Block 12 cDNA Libraries and Hybridization of Probes to DNA Libraries Normalization Pooling of cDNA libraries, 200ng each Sample μl cDNA B12T24J1 1.68 B12T24J2 1.60...
Australia Mytilus EecSeq Probe Synthesis Day 7
Restriction Digest of Adapters, Mung bean Nuclease Treatment, and Biotinylation Cleanup and visualization of probes were done the next day, but are included here for clarity Continuing with Amy Zyck...