Library Prep of pentagona HMW DNA for minION Sequencing
Using the Oxford Nanopore Ligation Sequencing Kit and the NEB Companion Module for Oxford Nanopore Technologies Ligation Sequencing on two HMW DNA pentagona samples Two samples were chosen, because of...
Second Extraction Pentagona HMW Size Selection with BluePippin
Using the BluePippin High Pass Plus Cassette, >15 kb DNA size collections to further refine the second HMW pentagona DNA extraction to above 15,000bp fragments only Sample Prep 11/12/19 Took...
More HMW Extractions on pentagona seastar with Zymo Quick-DNA HMW MagBead Kit
Using the Zymo Quick-DNA HMW MagBead Kit with the rest of one Cryptasterina pentagona tissue sample in DNA/RNA shield to try to get maximum HMW DNA!! Sample Prep Thawed PG...
Pentagona HMW Size Selection with BluePippin
Using the BluePippin High Pass Plus Cassette, >15 kb DNA size collections to further refine our HMW pentagona DNA to above 15,000bp fragments only Sample Prep 11/1/19 Took out electrophoresis...
Testing Zymo Quick-DNA HMW MagBead Kit on pentagona Seastar Tissue
Testing the Zymo Quick-DNA HMW MagBead Kit with one Cryptasterina pentagona tissue sample in DNA/RNA shield Sample Prep Added 260µl of Pro k storage buffer to the 5mg of powder...