Extracting DNA and RNA from Porites astreoides corals for genome project to use the RNA for new Zymo Ribo-free RNASeq test kit

Samples

G1-4
G1-13
Hot # 3 1/2 salinity
TA-Amb 1
TA-Amb 2
TA-HOT 2

Followed exact protocol Kevin Wong had done previously on other samples for these corals

Note: very hard to remove tissue from these corals, did not scrape or chip off easily and some were wet/mucousy even though they were taken quickly from the -80

Qubit, Gel and TapeStation

Qubit, gel, and tapestation were all done by Erin Chille on 8-20-19

Qubit:

all values are in ng/ul

Sample	DNA 1	DNA 2	RNA 1	RNA 2
G1-4	15	15	26.6	26.6
G1-13	15.5	15.5	27.8	26.6
Hot #3 1/2 salinity	8.84	8.82	18.8	18.6
TA-Amb 1	32.2	32.2	31.4	31.4
TA-Amb 2	33	32.8	31.4	31.6
TA-Hot 2	30.4	30.4	15.4	15.4

Gel

RNA TapeStation

Full results include a sperm sample from a different project