Fish2degCBL, Fish2CBR Primer (cytb-Fish), amplify 80 pb, Thomsen et al. 2012

PCR #1, Pag 61, Jul 23 2019

PCR reaction

• ~40 ng/ul DNA
• 1 ul primer (10um)
• Hoststar Qiagen 10 ul
• 1 ul BS
• 25 ul total reaction

Cycling conditions (Thomsen et al. 2012)

• 7 min to 95°C
• 50 cycles: 94°C 30 s, 50°C 30 s, 72°C 20 s,
• 5 min to 72°C
• 4°C forever

3ul gel green, 5ul PCR product, 4ul 1kb, 100 v, 2 hrs.

Slight amplification in some samples. IPT1, IPT2 and GSO1 samples will be taken to send to the test sequencing.

Fish2deg, PCR