Fish2deg-p77
Fish2degCBL, Fish2CBR Primer (cytb-Fish), amplify 80 pb, Thomsen et al. 2012
PCR #4, Pag 77, Aug 10 2019
PCR reaction
- ~40 ng/ul DNA
- 1 ul primer (10um)
- Hoststart Sahara (new) 10 ul
- 1.5 ul BSA
- 25 ul total reaction
Cycling conditions (Thomsen et al. 2012)
- 7 min to 95°C
- 50 cycles: 94°C 30 s, 50°C 30 s, 72°C 20 s,
- 5 min to 72°C
- 4°C forever
5ul PCR product 4ul 1kb 100 v, 2 hrs.
Contamination!. I used new reagents. I will not use BSA, it is possible Sahara Hotstart works better.