Extracting DNA of the second 8 samples from the March 2019 Sampling
Using Qiagen DNeasy Blood & Tissue kit and modifications from modifications from Renshaw et. al
| Sample | Location | Sampling/Filtering Date | Notes |
|---|---|---|---|
| CS-4-2 | Colt State Park | 03/23/19 | from the last 200mL of CS-4, Epoch column |
| MP-1 | Mill Pond | 03/23/19 | |
| MP-2 | Mill Pond | 03/23/19 | |
| MP-3 | Mill Pond | 03/23/19 | Epoch column |
| MP-4 | Mill Pond | 03/23/19 | |
| CF-1 | Common Fence Point | 03/23/19 | |
| CF-2 | Common Fence Point | 03/23/19 | |
| CF-3-8 | Common Fence Point | 03/23/19 | from the first 800mL of CF-4, Epoch column |
Followed exact extraction method of this post However 3 samples (CS-4-2, MP-3, and CF-3-8) were extracted with Epoch Science columns instead of the Qiagen column as a test.
Qubit was done with the Broad Range dsDNA kit
| Sample # | Standard 1 | Standard 2 | DNA 1 | DNA 2 |
|---|---|---|---|---|
| CS-4-2 | 204 | 21432 | 39.6 | 39.2 |
| MP-1 | 204 | 21432 | 29.6 | 29.4 |
| MP-2 | 204 | 21432 | 35.4 | 34.8 |
| MP-3 | 204 | 21432 | 57.0 | 56.8 |
| MP-4 | 204 | 21432 | 65.8 | 65.4 |
| CF-1 | 204 | 21432 | 86.4 | 86.4 |
| CF-2 | 204 | 21432 | 59.6 | 59.4 |
| CF-3-8 | 204 | 21432 | 47.6 | 47.6 |