2019-10-2-Fish2deg-p98
Fish2degCBL, Fish2CBR Primer (cytb-Fish), amplify 80 pb, Thomsen et al. 2012
PCR #6, Pag 98, Oct 2 2019
PCR reaction
- ~ 40 ng/ul DNA
- 0.5 ul primer (10um)
- 5 ul Hoststart Qiagen
- 12.5 ul total reaction
- 0.75 ul BSA
Cycling conditions (Thomsen et al. 2012)
- 7 min to 95°C
- 50 cycles: 94°C 30 s, 50°C 30 s, 72°C 20 s,
- 5 min to 72°C
- 4°C forever
Fragment sizes were verified on 1% agarose gels stained with GelRed 4ul PCR product 4ul 1kb 100 v, 2 hrs.
Amplification looks good in the gel, except to IPT2. The samples will be take to sequencing test.