Chord_Ceph_Tag_B_page_45

Jun 22, 2019

Testing Chord_Ceph_Tag_B with a random samples from the big project. Pag # 45.

For the master mix:

  1. 0.3 µl of each primer (F and R) = 2.7 µl each
  2. 5 µl of HotStart Pol = Total of 45 µl
  3. 2 µl nuclease free water, until you reach 10 ul total reaction
  4. 1.5 µl of DNA template (15 ng/ml); depending of the DNA concentration of the Sample
  5. 10 µl total reaction
  6. 8.5 µl of master mix was pipetted in the strip tube
  7. 1 µl of BSA
  8. I tested 7 samples and 1 negative
  9. 52 C using CHO52long program

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Gel: 1% gel was run with GelGreen for 1.5 hours at 110V

The samples doesn’t amplified well in this PCR.

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Chord_Ceph_Tag_B, PCR