Ceph_16S_page_40

Testing Ceph_16S with a NB samples from the small project. Pag # 40.

For the master mix:

1. 0.3 µl of each primer (F and R) = 2.7 µl each
2. 5 µl of HotStart Pol = Total of 45 µl
3. 0.4 µl nuclease free water, until you reach 10 ul total reaction
4. 4 µl of DNA template
5. 10 µl total reaction
6. 6 µl of master mix was pipetted in the strip tube
7. I tested 7 samples and 1 negative
8. 52 C using CHO52long program

Gel: 1% gel was run with GelGreen for 1.5 hours at 110V

The samples doesn’t amplified well in this PCR.

Ceph_16S, PCR