Chord_Ceph_Tag_B page 11
Testing Chord_Ceph_Tag_B. Pag # 11.
For the master mix:
- 0.5 µl of each primer (F and R) = 8.5 µl each
- 5 µl of Kapa HotStart = Total of 85 µl
- 5 µl nuclease free water, until you reach 25 ul total reaction
- 1 µl of DNA template (30ng/ml); depending of the DNA concentration of the Sample
- 12 µl total reaction
- 11 µl of master mix was pipetted in the strip tube
- I tested 14 samples and 2 negative
- 52 C
Gel 1: 1% gel was run with GelGreen for 1.5 hours at 110V
This PCR does not work
