PCR of Dinn-1 and Dv-1 Cells Inocculatd with DiNV PIF3 and Lambda Primers
Starting out using PIF3 (virus) and lambda primers for this qPCR, using samples that were extracted here
- Samples were kept on ice
- Reagents were thawed and kept on ice
- Master mixes for each primer were made and kept on ice until use
- Lambda master mix
- 505ul Sso
- 50.5ul Lambda F primer
- 50.5ul Lambda R primer
- 303ul molecular grade water
- PIF3 master mix
- 505ul Sso
- 50.5ul PIF3 F primer
- 50.5ul PIF3 R primer
- 303ul molecular grade water
- 9ul of primer was added to each well of a qPCR plate
- 1ul of diluted (or not) sample was added to the appropriate well in the plate
- The wells were pipette mixed 10X with a multichannel to mix
- The plate was sealed with the biorad seal
- The plate was spun down for 3 minutes at 4000rpm
- The plate was brought to the qPCR machine and run on the p47 program
Plate layouts can be found here, there was one plate for Lambda, and one plate for PIF 3
| well | sample | replicate | primer | cell_type | infection | day |
|---|---|---|---|---|---|---|
| A01 | 1 | 1 | PIF 3 | DV-1 | control | day0 |
| A02 | 1 | 2 | PIF 3 | DV-1 | control | day0 |
| A03 | 1 | 3 | PIF 3 | DV-1 | control | day0 |
| A04 | 2 | 1 | PIF 3 | DV-1 | control | day0 |
| A05 | 2 | 2 | PIF 3 | DV-1 | control | day0 |
| A06 | 2 | 3 | PIF 3 | DV-1 | control | day0 |
| A07 | 3 | 1 | PIF 3 | DV-1 | control | day0 |
| A08 | 3 | 2 | PIF 3 | DV-1 | control | day0 |
| A09 | 3 | 3 | PIF 3 | DV-1 | control | day0 |
| A10 | 4 | 1 | PIF 3 | DV-1 | control | day0 |
| A11 | 4 | 2 | PIF 3 | DV-1 | control | day0 |
| A12 | 4 | 3 | PIF 3 | DV-1 | control | day0 |
| B01 | 5 | 1 | PIF 3 | Dinn | control | day0 |
| B02 | 5 | 2 | PIF 3 | Dinn | control | day0 |
| B03 | 5 | 3 | PIF 3 | Dinn | control | day0 |
| B04 | 6 | 1 | PIF 3 | Dinn | control | day0 |
| B05 | 6 | 2 | PIF 3 | Dinn | control | day0 |
| B06 | 6 | 3 | PIF 3 | Dinn | control | day0 |
| B07 | 7 | 1 | PIF 3 | Dinn | control | day0 |
| B08 | 7 | 2 | PIF 3 | Dinn | control | day0 |
| B09 | 7 | 3 | PIF 3 | Dinn | control | day0 |
| B10 | 8 | 1 | PIF 3 | Dinn | control | day0 |
| B11 | 8 | 2 | PIF 3 | Dinn | control | day0 |
| B12 | 8 | 3 | PIF 3 | Dinn | control | day0 |
| C01 | 9 | 1 | PIF 3 | DV-1 | control | day5 |
| C02 | 9 | 2 | PIF 3 | DV-1 | control | day5 |
| C03 | 9 | 3 | PIF 3 | DV-1 | control | day5 |
| C04 | 10 | 1 | PIF 3 | DV-1 | control | day5 |
| C05 | 10 | 2 | PIF 3 | DV-1 | control | day5 |
| C06 | 10 | 3 | PIF 3 | DV-1 | control | day5 |
| C07 | 11 | 1 | PIF 3 | DV-1 | control | day5 |
| C08 | 11 | 2 | PIF 3 | DV-1 | control | day5 |
| C09 | 11 | 3 | PIF 3 | DV-1 | control | day5 |
| C10 | 12 | 1 | PIF 3 | DV-1 | control | day5 |
| C11 | 12 | 2 | PIF 3 | DV-1 | control | day5 |
| C12 | 12 | 3 | PIF 3 | DV-1 | control | day5 |
| D01 | 13 | 1 | PIF 3 | Dinn | control | day5 |
| D02 | 13 | 2 | PIF 3 | Dinn | control | day5 |
| D03 | 13 | 3 | PIF 3 | Dinn | control | day5 |
| D04 | 14 | 1 | PIF 3 | Dinn | control | day5 |
| D05 | 14 | 2 | PIF 3 | Dinn | control | day5 |
| D06 | 14 | 3 | PIF 3 | Dinn | control | day5 |
| D07 | 15 | 1 | PIF 3 | Dinn | control | day5 |
| D08 | 15 | 2 | PIF 3 | Dinn | control | day5 |
| D09 | 15 | 3 | PIF 3 | Dinn | control | day5 |
| D10 | 16 | 1 | PIF 3 | Dinn | control | day5 |
| D11 | 16 | 2 | PIF 3 | Dinn | control | day5 |
| D12 | 16 | 3 | PIF 3 | Dinn | control | day5 |
| E01 | 17 | 1 | PIF 3 | DV-1 | DiNV | day0 |
| E02 | 17 | 2 | PIF 3 | DV-1 | DiNV | day0 |
| E03 | 17 | 3 | PIF 3 | DV-1 | DiNV | day0 |
| E04 | 18 | 1 | PIF 3 | DV-1 | DiNV | day0 |
| E05 | 18 | 2 | PIF 3 | DV-1 | DiNV | day0 |
| E06 | 18 | 3 | PIF 3 | DV-1 | DiNV | day0 |
| E07 | 19 | 1 | PIF 3 | DV-1 | DiNV | day0 |
| E08 | 19 | 2 | PIF 3 | DV-1 | DiNV | day0 |
| E09 | 19 | 3 | PIF 3 | DV-1 | DiNV | day0 |
| E10 | 20 | 1 | PIF 3 | DV-1 | DiNV | day0 |
| E11 | 20 | 2 | PIF 3 | DV-1 | DiNV | day0 |
| E12 | 20 | 3 | PIF 3 | DV-1 | DiNV | day0 |
| F01 | 21 | 1 | PIF 3 | Dinn | DiNV | day0 |
| F02 | 21 | 2 | PIF 3 | Dinn | DiNV | day0 |
| F03 | 21 | 3 | PIF 3 | Dinn | DiNV | day0 |
| F04 | 22 | 1 | PIF 3 | Dinn | DiNV | day0 |
| F05 | 22 | 2 | PIF 3 | Dinn | DiNV | day0 |
| F06 | 22 | 3 | PIF 3 | Dinn | DiNV | day0 |
| F07 | 23 | 1 | PIF 3 | Dinn | DiNV | day0 |
| F08 | 23 | 2 | PIF 3 | Dinn | DiNV | day0 |
| F09 | 23 | 3 | PIF 3 | Dinn | DiNV | day0 |
| F10 | 24 | 1 | PIF 3 | Dinn | DiNV | day0 |
| F11 | 24 | 2 | PIF 3 | Dinn | DiNV | day0 |
| F12 | 24 | 3 | PIF 3 | Dinn | DiNV | day0 |
| G01 | 25 | 1 | PIF 3 | DV-1 | DiNV | day5 |
| G02 | 25 | 2 | PIF 3 | DV-1 | DiNV | day5 |
| G03 | 25 | 3 | PIF 3 | DV-1 | DiNV | day5 |
| G04 | 26 | 1 | PIF 3 | DV-1 | DiNV | day5 |
| G05 | 26 | 2 | PIF 3 | DV-1 | DiNV | day5 |
| G06 | 26 | 3 | PIF 3 | DV-1 | DiNV | day5 |
| G07 | 27 | 1 | PIF 3 | DV-1 | DiNV | day5 |
| G08 | 27 | 2 | PIF 3 | DV-1 | DiNV | day5 |
| G09 | 27 | 3 | PIF 3 | DV-1 | DiNV | day5 |
| G10 | 28 | 1 | PIF 3 | DV-1 | DiNV | day5 |
| G11 | 28 | 2 | PIF 3 | DV-1 | DiNV | day5 |
| G12 | 28 | 3 | PIF 3 | DV-1 | DiNV | day5 |
| H01 | 29 | 1 | PIF 3 | Dinn | DiNV | day5 |
| H02 | 29 | 2 | PIF 3 | Dinn | DiNV | day5 |
| H03 | 29 | 3 | PIF 3 | Dinn | DiNV | day5 |
| H04 | 30 | 1 | PIF 3 | Dinn | DiNV | day5 |
| H05 | 30 | 2 | PIF 3 | Dinn | DiNV | day5 |
| H06 | 30 | 3 | PIF 3 | Dinn | DiNV | day5 |
| H07 | 31 | 1 | PIF 3 | Dinn | DiNV | day5 |
| H08 | 31 | 2 | PIF 3 | Dinn | DiNV | day5 |
| H09 | 31 | 3 | PIF 3 | Dinn | DiNV | day5 |
| H10 | 32 | 1 | PIF 3 | Dinn | DiNV | day5 |
| H11 | 32 | 2 | PIF 3 | Dinn | DiNV | day5 |
| H12 | 32 | 3 | PIF 3 | Dinn | DiNV | day5 |
| A01 | 1 | 1 | LAMBDA | DV-1 | control | day0 |
| A02 | 1 | 2 | LAMBDA | DV-1 | control | day0 |
| A03 | 1 | 3 | LAMBDA | DV-1 | control | day0 |
| A04 | 2 | 1 | LAMBDA | DV-1 | control | day0 |
| A05 | 2 | 2 | LAMBDA | DV-1 | control | day0 |
| A06 | 2 | 3 | LAMBDA | DV-1 | control | day0 |
| A07 | 3 | 1 | LAMBDA | DV-1 | control | day0 |
| A08 | 3 | 2 | LAMBDA | DV-1 | control | day0 |
| A09 | 3 | 3 | LAMBDA | DV-1 | control | day0 |
| A10 | 4 | 1 | LAMBDA | DV-1 | control | day0 |
| A11 | 4 | 2 | LAMBDA | DV-1 | control | day0 |
| A12 | 4 | 3 | LAMBDA | DV-1 | control | day0 |
| B01 | 5 | 1 | LAMBDA | Dinn | control | day0 |
| B02 | 5 | 2 | LAMBDA | Dinn | control | day0 |
| B03 | 5 | 3 | LAMBDA | Dinn | control | day0 |
| B04 | 6 | 1 | LAMBDA | Dinn | control | day0 |
| B05 | 6 | 2 | LAMBDA | Dinn | control | day0 |
| B06 | 6 | 3 | LAMBDA | Dinn | control | day0 |
| B07 | 7 | 1 | LAMBDA | Dinn | control | day0 |
| B08 | 7 | 2 | LAMBDA | Dinn | control | day0 |
| B09 | 7 | 3 | LAMBDA | Dinn | control | day0 |
| B10 | 8 | 1 | LAMBDA | Dinn | control | day0 |
| B11 | 8 | 2 | LAMBDA | Dinn | control | day0 |
| B12 | 8 | 3 | LAMBDA | Dinn | control | day0 |
| C01 | 9 | 1 | LAMBDA | DV-1 | control | day5 |
| C02 | 9 | 2 | LAMBDA | DV-1 | control | day5 |
| C03 | 9 | 3 | LAMBDA | DV-1 | control | day5 |
| C04 | 10 | 1 | LAMBDA | DV-1 | control | day5 |
| C05 | 10 | 2 | LAMBDA | DV-1 | control | day5 |
| C06 | 10 | 3 | LAMBDA | DV-1 | control | day5 |
| C07 | 11 | 1 | LAMBDA | DV-1 | control | day5 |
| C08 | 11 | 2 | LAMBDA | DV-1 | control | day5 |
| C09 | 11 | 3 | LAMBDA | DV-1 | control | day5 |
| C10 | 12 | 1 | LAMBDA | DV-1 | control | day5 |
| C11 | 12 | 2 | LAMBDA | DV-1 | control | day5 |
| C12 | 12 | 3 | LAMBDA | DV-1 | control | day5 |
| D01 | 13 | 1 | LAMBDA | Dinn | control | day5 |
| D02 | 13 | 2 | LAMBDA | Dinn | control | day5 |
| D03 | 13 | 3 | LAMBDA | Dinn | control | day5 |
| D04 | 14 | 1 | LAMBDA | Dinn | control | day5 |
| D05 | 14 | 2 | LAMBDA | Dinn | control | day5 |
| D06 | 14 | 3 | LAMBDA | Dinn | control | day5 |
| D07 | 15 | 1 | LAMBDA | Dinn | control | day5 |
| D08 | 15 | 2 | LAMBDA | Dinn | control | day5 |
| D09 | 15 | 3 | LAMBDA | Dinn | control | day5 |
| D10 | 16 | 1 | LAMBDA | Dinn | control | day5 |
| D11 | 16 | 2 | LAMBDA | Dinn | control | day5 |
| D12 | 16 | 3 | LAMBDA | Dinn | control | day5 |
| E01 | 17 | 1 | LAMBDA | DV-1 | DiNV | day0 |
| E02 | 17 | 2 | LAMBDA | DV-1 | DiNV | day0 |
| E03 | 17 | 3 | LAMBDA | DV-1 | DiNV | day0 |
| E04 | 18 | 1 | LAMBDA | DV-1 | DiNV | day0 |
| E05 | 18 | 2 | LAMBDA | DV-1 | DiNV | day0 |
| E06 | 18 | 3 | LAMBDA | DV-1 | DiNV | day0 |
| E07 | 19 | 1 | LAMBDA | DV-1 | DiNV | day0 |
| E08 | 19 | 2 | LAMBDA | DV-1 | DiNV | day0 |
| E09 | 19 | 3 | LAMBDA | DV-1 | DiNV | day0 |
| E10 | 20 | 1 | LAMBDA | DV-1 | DiNV | day0 |
| E11 | 20 | 2 | LAMBDA | DV-1 | DiNV | day0 |
| E12 | 20 | 3 | LAMBDA | DV-1 | DiNV | day0 |
| F01 | 21 | 1 | LAMBDA | Dinn | DiNV | day0 |
| F02 | 21 | 2 | LAMBDA | Dinn | DiNV | day0 |
| F03 | 21 | 3 | LAMBDA | Dinn | DiNV | day0 |
| F04 | 22 | 1 | LAMBDA | Dinn | DiNV | day0 |
| F05 | 22 | 2 | LAMBDA | Dinn | DiNV | day0 |
| F06 | 22 | 3 | LAMBDA | Dinn | DiNV | day0 |
| F07 | 23 | 1 | LAMBDA | Dinn | DiNV | day0 |
| F08 | 23 | 2 | LAMBDA | Dinn | DiNV | day0 |
| F09 | 23 | 3 | LAMBDA | Dinn | DiNV | day0 |
| F10 | 24 | 1 | LAMBDA | Dinn | DiNV | day0 |
| F11 | 24 | 2 | LAMBDA | Dinn | DiNV | day0 |
| F12 | 24 | 3 | LAMBDA | Dinn | DiNV | day0 |
| G01 | 25 | 1 | LAMBDA | DV-1 | DiNV | day5 |
| G02 | 25 | 2 | LAMBDA | DV-1 | DiNV | day5 |
| G03 | 25 | 3 | LAMBDA | DV-1 | DiNV | day5 |
| G04 | 26 | 1 | LAMBDA | DV-1 | DiNV | day5 |
| G05 | 26 | 2 | LAMBDA | DV-1 | DiNV | day5 |
| G06 | 26 | 3 | LAMBDA | DV-1 | DiNV | day5 |
| G07 | 27 | 1 | LAMBDA | DV-1 | DiNV | day5 |
| G08 | 27 | 2 | LAMBDA | DV-1 | DiNV | day5 |
| G09 | 27 | 3 | LAMBDA | DV-1 | DiNV | day5 |
| G10 | 28 | 1 | LAMBDA | DV-1 | DiNV | day5 |
| G11 | 28 | 2 | LAMBDA | DV-1 | DiNV | day5 |
| G12 | 28 | 3 | LAMBDA | DV-1 | DiNV | day5 |
| H01 | 29 | 1 | LAMBDA | Dinn | DiNV | day5 |
| H02 | 29 | 2 | LAMBDA | Dinn | DiNV | day5 |
| H03 | 29 | 3 | LAMBDA | Dinn | DiNV | day5 |
| H04 | 30 | 1 | LAMBDA | Dinn | DiNV | day5 |
| H05 | 30 | 2 | LAMBDA | Dinn | DiNV | day5 |
| H06 | 30 | 3 | LAMBDA | Dinn | DiNV | day5 |
| H07 | 31 | 1 | LAMBDA | Dinn | DiNV | day5 |
| H08 | 31 | 2 | LAMBDA | Dinn | DiNV | day5 |
| H09 | 31 | 3 | LAMBDA | Dinn | DiNV | day5 |
| H10 | 32 | 1 | LAMBDA | Dinn | DiNV | day5 |
| H11 | 32 | 2 | LAMBDA | Dinn | DiNV | day5 |
| H12 | 32 | 3 | LAMBDA | Dinn | DiNV | day5 |