- This is 48 hours after inoculation
- Fresh 80% acetone for fixing had already been made up and stored at -20C
- Acetone then placed on ice for use
- Two 48 well plates of inoculated Dv-1 cells were dumped out onto paper towels and tapped down (towels were disposed of in autoclave waste)
- Slowly dripped with serological pipette the 80% acetone into each well that had cells until each well was full
- Placed plates in -20 freezer for 30 minutes
- Tapped and dumped out plates again to remove the acetone
- Let the plates dry with the lid open for 20 minutes
- Placed the plates covered in the -20 freezer overnight