Making Wash and Blocking Solutions for DiNV Titering with Already Plated Dv-1 Cells

80% acetone

  • 2 50mL conicals made
  • 40mL optima acetone
  • 10mL molecular grade water
  • Stored a -20

Blocking buffer

  • DPBS with 0.01% tween and 1% BSA
  • Made 2 150mL bottles that were 0.22um sterile filtered
  • 1.5g BSA powder (from 4012 fridge)
  • 150mL DPBS
  • 150ul tween solution
  • Mixed in larger beaker then sterile filtered into two bottles
  • Stored at 4C

Conjugate solution

  • DPBS with 1% BSA
  • 1 50mL conical
  • 0.5g BSA powder
  • 50mL DPBS
  • Mixed in 50mL conical and vortexed to mix
  • Sterile filtered 0.22um
  • Stored at 4C

Wash solution was DPBS stored at 4C