Incoulating 3 Replicate Dinn Cell Flasks for Monitoring DiNV Evolution over 3 Months
Cells are 3 replicate flasks of Dinn3 cells that were plated here and tested for virus here. Samples wre virus negative.
Plan for whole experiment is here
All processes were done in the cell culture hood and with sterile technique
- A 200ul vial of fly homogenate #5 was thawed on ice and vortexed
- 2 samples of 50ul of supernatant was taken from each flask for a day 0 sample (see table below)
- For each flask, 2.3mL of supernatant was removed and discarded
- Then, 5mL of new 10% FBS, 4% mushroom extract Schneider’s Medium with antibiotics was added to each flask
- 50ul of fly homogenate was added to each flask, and after adding each flask was gently rocked back and forth gently for 1 minute
- The remaining ~50ul in the tube was saved as a fly homogenate sample and frozen along with the other samples
- All 1.5mL tubes were frozen at -20C
- Flasks were placed in the back of the 23C incubator and will be left for 3 weeks (and probably checked on once a week)
| Sample # | Flask it came from |
|---|---|
| 4 | fly homogenate tube |
| 5 | A |
| 6 | A |
| 7 | B |
| 8 | B |
| 9 | C |
| 10 | C |