Australia Mytilus EecSeq Probe Synthesis Day 1
Planning, Pooling, and Concentrating RNA for EecSeq Probe Synthesis All steps in are done with Amy Zyck First step: Aliquoit out 1ug of RNA each from 4 samples from each...
Pooling CASE Block 11 cDNA Libraries for Sequencing
Pooling CASE Block 11 larval oyster cDNA from mRNA Libraries of T24 samples (made in November) for sequencing with Novogene company. Quants of libraries were calculated from the TapeStation traces...
Mytilus Live Tissue Tri-Reagent RNA Extraction
Extracting RNA from Live Mytilus edulis tissue using a Tri-Reagent/Direct-zol RNA Isolation Protocol written by Johanna Harvey Zymo Research Direct-zol RNA Miniprep Plus Kit Prep Doing 4 samples as a...
dsDNA and RNA Qubit Quantification Protocol
Broad Range dsDNA, High Sensitivity dsDNA, Broad Range RNA, and High Sensitivity RNA All Steps are the same whichever quantification method you are using Determine n number for calculating working...
PPP Lab Agarose Gel Protocol
Determine the size gel you need to make, remember to account for at least one well for a ladder on each row: Small gel comb capability: two combs with 14...