General Template For Sample Processing Notebook Post

Specific Title Pertaining to Method and Samples Processed

Include a Link to the protocol (from your lab notebook) used for processing these samples. Also include links and details for anything you used that is different from that post.

Goal for Processing: What was your goal for the day?
Major Results What is your major result with respect to your goal?
Major Take Aways Are there any broader conclusions that came from your labwork today, or links to other posts?

Components

  • List/table of samples that you processed this day
  • Mention all things that went differently in this day’s processing that aren’t in the referenced protocol
  • Note if a sample is a re-processing or not
  • If you are making a library, provide a table of the multiplexing done, and include the index/barcode sequences if available
  • If you are putting something in a 96 well plate for the plate reader, include a plate map of where your samples went

QC/Data Acquired

  • List all QC measures performed for this processing, if you have links to posts about their protocols, include them here.
  • Provide all data for QC ex: Qubit table, gel image, volumes of homogenized tissue, plate reader outputs, tapestation reports

Next Steps

  • Will you be re-doing some samples?
  • Are there many more samples to process in the same way going forward?
  • Are you going to be scaling up the processing level once you are comfortable with the protocol?
  • Etc etc etc
Written on March 26, 2020