Dilute, Re-Qunatify, and Re-Tape 1st KBay WGBS

Dilute and Re-QC WGBS Libraries from This Post Because of High Concentration and Potential Overloading of the TapeStation

Goal Add extra volume of elution buffer and re-do QC metrics
Results Concentrations are still high, tape looks slightly better/easier to read Takeaways I plan to now elute all KAPA-2nd amp libraries with 35ul elution buffer after the bead cleanup

I took the existing libraries, thawed them, and added 16ul of DNA elution buffer to each

Then I re-did the Qubit (concentration had changed), and the TapeStation.

Broad Range dsDNA Qubit

Sample Reading 1 (ng/ul) Reading 2(ng/ul) Average (ng/ul)
S1 179 RFU - -
S2 19028 RFU - -
21 33.2 32.8 33
22 31.2 31 31.1
26 31.2 31 31.1
28 29.6 29.4 29.5
55 29.4 29.2 29.3
30 30.2 39.8 30
44 27.8 27.8 27.8
51 29 29 29
31 12.3 12.3 12.3
2 26.6 26.6 26.6
38 28.6 28.4 28.5
32 24 24 24

D5000 TapeStation

Written on May 13, 2021