Injecting Male and Female D. innubila with DiNV for Kent and Gut Dissection 3
This time both Kent and Rob wanted flies, some for sectioning and some for gut dissection
- Flies had emerged and were separated out the day before, should be 5-7 days old
- All flies got undiluted P4 DiNV
- 14 new vials of food were made
- Used virus aliquot 37
- Infection followed exactly the nanoinjection protocol
- Fly mortality was not monitored because they were taken for timepoint dissections
| vial | sex | treatment | hour | type | time | time on | N# |
|---|---|---|---|---|---|---|---|
| 1 | F | Co2 only | 72 | section | 9:08 | 2min | 11 |
| 2 | M | CCM | 72 | IFA | 9:31 | 5min | 9 |
| 3 | F | CCM | 72 | IFA | 9:37 | 5min | 10 |
| 4 | F | CCM | 72 | section | 9:42 | 5min | 10 |
| 5 | F | P4 DiNV | 48 | IFA | 9:55 | 5min | 11 |
| 6 | F | P4 DiNV | 72 | IFA | 10:00 | 5min | 9 |
| 7 | F | P4 DiNV | 72 | section | 10:05 | 5min | 11 |
| 8 | F | P4 DiNV | 96 | IFA | 10:11 | 5min | 10 |
| 9 | M | P4 DiNV | 72 | IFA | 10:16 | 5min | 10 |