Injecting Male and Female D. innubila with DiNV for Kent and Gut Dissection 2
This time both Kent and Rob wanted flies, some for sectioning and some for gut dissection
- Flies had emerged and were separated out the day before, should be 5-7 days old
- All flies got undiluted P4 DiNV
- 14 new vials of food were made
- Used virus aliquot 36
- Infection followed exactly the nanoinjection protocol
- Fly mortality was not monitored because they were taken for timepoint dissections
| vial | sex | treatment | for | day/hour | time | time on | N# |
|---|---|---|---|---|---|---|---|
| 1 | M | NA | Rob | 0 | 9:18 | 2min | 8 |
| 2 | M | NA | Rob | 72 | 9:18 | 2min | 9 |
| 3 | F | NA | Rob | 72 | 9:37 | 2min | 9 |
| 4 | F | NA | Rob | 0 | 9:37 | 2min | 8 |
| 5 | M | CCM | Kent | 72 | 9:22 | 5min | 10 |
| 6 | F | CCM | Kent | 72 | 9:27 | 5min | 10 |
| 7 | F | P4 DiNV | Rob | 6 | 9:45 | 7min | 7 |
| 8 | F | P4 DiNV | Rob | 12 | 9:45 | 7min | 7 |
| 9 | F | P4 DiNV | Rob | 24 | 9:53 | 8min | 8 |
| 10 | F | P4 DiNV | Rob | 48 | 9:53 | 8min | 8 |
| 11 | F | P4 DiNV | Rob | 72 | 10:02 | 4min | 9 |
| 12 | M | P4 DiNV | Rob | 72 | 10:05 | 4min | 9 |
| 13 | F | P4 DiNV | Kent | 72 | 10:11 | 5min | 10 |
| 14 | M | P4 DiNV | Kent | 72 | 10:16 | 5min | 11 |