Plating Dinn-1 Cells for Infection then RNA
I planned to do these in 24 well plates, and have 1 plate per day that needs to be frozen so I don’t need to disturb plates that are not being used on certain days.
- Each well needs ~1mL of cells and fluid
- I cell scraped a flask of Dinn-1 cells
- Pelleted the cells and removed the supernatant
- Resuspended the cells in 30mL of fresh medium
- Added 1mL of cell solution to each well in the planned 24 well plates
- These plates were put in the incubator until infection so they could grow out
- Outer wells were filled with water to prevent evaporation
Plate layouts:
Day 0:
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | ||||||
| B | C | C | C | C | ||
| C | ||||||
| D |
Day 1:
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | ||||||
| B | C | C | C | C | ||
| C | V | V | V | V | ||
| D |
Day 3:
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | ||||||
| B | C | C | C | C | ||
| C | V | V | V | V | ||
| D |
Day 5:
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | ||||||
| B | C | C | C | C | ||
| C | V | V | V | V | ||
| D |