Infecting 24 Well Plates of Dv-1 and Myd88 Cells with DiNV to See if Myd88 Cells Grow Virus
Cells were already plated the day before and have settled to the plates.
There is a plate for day 0 just after inoculation and a plate for day 5 after inoculation.
Plate layout:
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | Dv-1 CC | Myd88 CC | X | Dv-1 DiNV | Myd88 DiNV | X |
| B | Dv-1 CC | Myd88 CC | X | Dv-1 DiNV | Myd88 DiNV | X |
| C | Dv-1 CC | Myd88 CC | X | Dv-1 DiNV | Myd88 DiNV | X |
| D | Dv-1 CC | Myd88 CC | X | Dv-1 DiNV | Myd88 DiNV | X |
- I wanted to add 10ul of passage 4 virus to each well that gets infected, but I wanted the volume added to be larger to mix more
- So I diluted 250ul of P4 DiNV with 375ul of medium
- Then added 25ul of that solution to each virus well
- And 25ul of straight medium to the control wells
- Rocked the plates back and forth a bit
- Day 0 plate was placed in the -80 immediately
- The day 5 plate was placed in the 23C incubator for 5 days, when it was also placed in the -80