Set 2 of DNA Extracts of Male D. innubila From DiNV Infections from 01/31 and PCRs
All samples were thawed on ice, this general protocol was used, and filter tips were used at all times.
| tube | replicate | treatment | day frozen | dead? |
|---|---|---|---|---|
| ex ctrl 3 | A | NA | NA | NA |
| 22 | A | CCM | day12 | yes |
| 38 | A | CCM | day14 | no |
| 39 | A | CCM | day14 | no |
| 40 | A | CCM | day14 | no |
| 41 | A | CCM | day14 | no |
| 42 | A | CCM | day14 | no |
| 43 | A | CCM | day14 | no |
| 44 | A | CCM | day14 | no |
| 45 | A | CCM | day14 | no |
| 46 | A | CCM | day14 | no |
| 1 | A | 0.005 FFU | day3 | yes |
| 34 | A | 0.01 FFU | day12 | yes |
| 35 | A | 0.01 FFU | day12 | yes |
| 36 | A | 0.01 FFU | day12 | yes |
| 37 | A | 0.01 FFU | day12 | yes |
| 47 | A | 0.005 FFU | day14 | no |
| 48 | A | 0.005 FFU | day14 | no |
| 49 | A | 0.005 FFU | day14 | no |
| 50 | A | 0.005 FFU | day14 | no |
| 51 | A | 0.005 FFU | day14 | no |
| 52 | A | 0.005 FFU | day14 | no |
| 53 | A | 0.005 FFU | day14 | no |
| 54 | A | 0.005 FFU | day14 | no |
| 55 | A | 0.005 FFU | day14 | no |
| 56 | A | 0.005 FFU | day14 | no |
| 57 | A | 0.005 FFU | day14 | no |
| 58 | A | 0.005 FFU | day14 | no |
| 63 | A | 0.005 FFU | day14 | no |
| 64 | A | 0.005 FFU | day14 | no |
| 65 | A | 0.005 FFU | day14 | no |
| 77 | A | 0.01 FFU | day14 | no |
| 34 | A | 0.01 FFU | day12 | yes |
| 35 | A | 0.01 FFU | day12 | yes |
| 36 | A | 0.01 FFU | day12 | yes |
| 37 | A | 0.01 FFU | day12 | yes |
PCRs
- PCRs were run on the samples, and the process followed the general PCR protocol completely.
- Primer information can be found here
- PCRs were then run on 1% gels for 35 minutes at 90V
Information and scoring on these samples can be found here