Set 1 and 2 of DNA Extracts of Male D. innubila From DiNV Infections from 02/21 and PCRs
All samples were thawed on ice, this general protocol was used, and filter tips were used at all times. I did two sets of extractions, one set of 48 then the remaining 24.
Set of 48:
| tube | replicate | sex | treatment | day | dead |
|---|---|---|---|---|---|
| ex ctrl 1 | B | NA | NA | Na | NA |
| 6 | B | male | CCM | day6 | yes |
| 8 | B | male | CCM | day6 | yes |
| 25 | B | male | CCM | day9 | yes |
| 54 | B | male | CCM | day12 | yes |
| 1 | B | male | 0.005 FFU | day3 | yes |
| 2 | B | male | 0.005 FFU | day3 | yes |
| 5 | B | male | 0.05 FFU | day3 | yes |
| 10 | B | male | 0.005 FFU | day6 | yes |
| 12 | B | male | 0.005 FFU | day6 | yes |
| 13 | B | male | 0.01 FFU | day6 | yes |
| 14 | B | male | 0.01 FFU | day6 | yes |
| 17 | B | male | 0.01 FFU | day6 | yes |
| 20 | B | male | 0.05 FFU | day6 | yes |
| 23 | B | male | 0.05 FFU | day6 | yes |
| 31 | B | male | 0.005 FFU | day9 | yes |
| 32 | B | male | 0.005 FFU | day9 | yes |
| 33 | B | male | 0.005 FFU | day9 | yes |
| 37 | B | male | 0.01 FFU | day9 | yes |
| 38 | B | male | 0.01 FFU | day9 | yes |
| 40 | B | male | 0.05 FFU | day9 | yes |
| 98 | B | male | 0.005 FFU | day14 | no |
| 46 | B | male | 0.05 FFU | day9 | yes |
| 47 | B | male | 0.05 FFU | day9 | yes |
| 48 | B | male | 0.05 FFU | day9 | yes |
| 55 | B | male | 0.005 FFU | day12 | yes |
| 63 | B | male | 0.05 FFU | day12 | yes |
| 64 | B | male | 0.05 FFU | day12 | yes |
| 65 | B | male | 0.05 FFU | day12 | yes |
| 118 | B | male | 0.01 FFU | day14 | no |
| 119 | B | male | 0.01 FFU | day14 | no |
| 120 | B | male | 0.01 FFU | day14 | no |
| 125 | B | male | 0.01 FFU | day14 | no |
| 126 | B | male | 0.01 FFU | day14 | no |
| 127 | B | male | 0.01 FFU | day14 | no |
| 128 | B | male | 0.01 FFU | day14 | no |
| 129 | B | male | 0.01 FFU | day14 | no |
| 130 | B | male | 0.01 FFU | day14 | no |
| 131 | B | male | 0.01 FFU | day14 | no |
| 132 | B | male | 0.01 FFU | day14 | no |
| 136 | B | male | 0.05 FFU | day14 | no |
| 137 | B | male | 0.05 FFU | day14 | no |
| 138 | B | male | 0.05 FFU | day14 | no |
| 140 | B | male | 0.05 FFU | day14 | no |
| 144 | B | male | 0.005 FFU | day14 | yes |
| 146 | B | male | 0.005 FFU | day14 | yes |
| 148 | B | male | 0.05 FFU | day14 | yes |
| ex ctrl 2 | B | NA | NA | NA | NA |
Set of 24:
| tube | replicate | sex | treatment | day | dead |
|---|---|---|---|---|---|
| ex ctrl 3 | B | NA | NA | NA | NA |
| 67 | B | male | CCM | day14 | no |
| 68 | B | male | CCM | day14 | no |
| 69 | B | male | CCM | day14 | no |
| 70 | B | male | CCM | day14 | no |
| 71 | B | male | CCM | day14 | no |
| 72 | B | male | CCM | day14 | no |
| 73 | B | male | CCM | day14 | no |
| 82 | B | male | CCM | day14 | no |
| 83 | B | male | CCM | day14 | no |
| 84 | B | male | CCM | day14 | no |
| 85 | B | male | CCM | day14 | no |
| 86 | B | male | CCM | day14 | no |
| 87 | B | male | CCM | day14 | no |
| 88 | B | male | CCM | day14 | no |
| 89 | B | male | CCM | day14 | no |
| 99 | B | male | 0.005 FFU | day14 | no |
| 100 | B | male | 0.005 FFU | day14 | no |
| 101 | B | male | 0.005 FFU | day14 | no |
| 102 | B | male | 0.005 FFU | day14 | no |
| 103 | B | male | 0.005 FFU | day14 | no |
| 104 | B | male | 0.005 FFU | day14 | no |
| 111 | B | male | 0.005 FFU | day14 | no |
| 112 | B | male | 0.005 FFU | day14 | no |
20240311 PCRs
- PCRs were run on the samples, and the process followed the general PCR protocol completely.
- Primer information can be found here
- PCRs were then run on 1% gels for 35 minutes at 90V
Set 1
Set 2 (also some CO1 from the replicate 1 of this experiment)
Information and scoring on these samples can be found here