Infecting Male D. innubila with Middle Band DiNV to Check Titering Accuracy Replicate 2
I used the same calculations as replicate 1 for diluting the MB virus stock
The virus solutions were kept on ice and pipette mixed
| tube | dilution for delivery | ul of virus solution | virus solution tube | ul of medium |
|---|---|---|---|---|
| 1 | 3 FFU | 12.35ul | stock tube 43 | 47.65ul |
| 2 | 1 FFU | 15ul | tube 1 | 30ul |
| 3 | 0.1 FFU | 5ul | tube 2 | 45ul |
| 4 | 0.05 FFU | 20ul | tube 3 | 20ul |
| 5 | 0.01 FFU | 5ul | tube 3 | 45ul |
Note that tube 3 was only used for making the dilutions, not for infections
- Flies had emerged on 2/28 and 2/29 and are 5-6 days old
- The males from those flies had been separated out the day before into 10 vials of ~10 males
- Infections followed the nanoinjection protocol
| vial | species | sex | mated status | days_emerged | day_infected | age_infected | tube | treatment | virus | volume | time | time on CO2 | original_N_number |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | CCM | CCM | middle band | 27.6nl | 4:30 | 5 min | 10 |
| 2 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | CCM | CCM | middle band | 27.6nl | 4:37 | 5 min | 10 |
| 3 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 5 | 0.01 | middle band | 27.6nl | 4:44 | 5 min | 10 |
| 4 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 5 | 0.01 | middle band | 27.6nl | 4:49 | 5 min | 10 |
| 5 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 4 | 0.05 | middle band | 27.6nl | 4:56 | 5 min | 10 |
| 6 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 4 | 0.05 | middle band | 27.6nl | 5:02 | 5 min | 10 |
| 7 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 2 | 1 | middle band | 27.6nl | 5:09 | 5 min | 10 |
| 8 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 2 | 1 | middle band | 27.6nl | 5:14 | 5 min | 10 |
| 9 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 1 | 3 | middle band | 27.6nl | 5:21 | 5 min | 10 |
| 10 | D. innubila | male | yes | 2/28 - 2/29 | 20240305 | 5-6 days | 1 | 3 | middle band | 27.6nl | 5:26 | 5 min | 10 |
Infection monitoring can be found here
Frozen fly information can be found here
Analysis can be found here, note that this is replicates combined