Set 1 of DNA Extracts of Male D. innubila From DiNV Infections from 01/31

All samples were thawed on ice, this general protocol was used, and filter tips were used at all times. At first I only extracted from flies who had died during the experiment, but later on I ended up extracting them all.

tube replicate treatment day frozen dead?
ex ctrl 1 A NA NA NA
2 A 0.05 FFU day3 yes
3 A 0.05 FFU day3 yes
4 A 0.005 FFU day6 yes
5 A 0.005 FFU day6 yes
6 A 0.01 FFU day6 yes
7 A 0.05 FFU day6 yes
8 A 0.05 FFU day6 yes
9 A 0.005 FFU day9 yes
10 A 0.005 FFU day9 yes
11 A 0.01 FFU day9 yes
12 A 0.05 FFU day9 yes
13 A 0.05 FFU day9 yes
14 A 0.05 FFU day9 yes
15 A 0.05 FFU day9 yes
16 A 0.05 FFU day9 yes
17 A 0.05 FFU day9 yes
18 A 0.05 FFU day9 yes
19 A 0.01 FFU day9 yes
20 A 0.01 FFU day9 yes
21 A 0.01 FFU day9 yes
23 A 0.005 FFU day12 yes
24 A 0.005 FFU day12 yes
25 A 0.01 FFU day12 yes
26 A 0.01 FFU day12 yes
27 A 0.05 FFU day12 yes
28 A 0.05 FFU day12 yes
29 A 0.05 FFU day12 yes
30 A 0.05 FFU day12 yes
31 A 0.01 FFU day12 yes
32 A 0.01 FFU day12 yes
33 A 0.01 FFU day12 yes
59 A 0.005 FFU day14 no
60 A 0.005 FFU day14 no
61 A 0.005 FFU day14 no
62 A 0.005 FFU day14 no
66 A 0.005 FFU day14 yes
67 A 0.01 FFU day14 no
68 A 0.01 FFU day14 no
69 A 0.01 FFU day14 no
70 A 0.01 FFU day14 no
71 A 0.01 FFU day14 no
72 A 0.01 FFU day14 no
73 A 0.05 FFU day14 no
74 A 0.05 FFU day14 no
75 A 0.05 FFU day14 no
76 A 0.05 FFU day14 no
ex ctrl 2 A NA NA NA

Then, I did PCR for p47 and TPI on each sample:

reagent P47 TPI
GoTaq 270ul 270ul
F primer 13.5ul 13.5ul
R primer 13.5ul 13.5ul
molec grade water 189ul 189ul
  • All mixes and DNA were kept on ice and vortexed and spun down before use, samples were run for 30 cycles

Results from the PCRs can be found here