Single Fly DNA extracts and TPI and p47 PCRs on day0 D. innubila that either got poked or injected with DiNV
Samples are from This infection
Samples were single flies in tubes, and had their DNA extracted with the DiNV specific DNA extraction protocol that takes care to minimize contamination and includes two extraction controls in each round of extraction. Information on the order of samples and their extraction dates can be found in this spreadsheet
After all samples had their DNA extracted, each sample went through PCR for TPI and p47. The general PCR protocol was followed, and the primer information can be found here. The p47 program was for 30 cycles. After, all samples were run a 1% gel for 35 minutes at 90V.
Gel image:
