Testing Concentrated DiNV Fluid with D. innubila Infections Round 1
In this project Huan and I are testing the concentrated fluid from DinnDiNV 4 cells on both D. innubila and D. falleni. He will do 2 infections on falleni and 1 on innubila, and I will do 2 infections on innbila and 1 on falleni.
Infection liquid is from the ultrafiltration concentrated fluids and the control liquid is complete cell culture medium (including 20% FBS and antibiotics) that was concentrated in the same way as the viral fluid (20 minute ultrafiltration spin). Information on tubes of concentrated fluid can be found here
Using the D. innubila DiNV infection protocol as the process for these infections.
Flies emerged between 3/22 and 3/23. This means they are 5-6 days old for this infection. The day before, 6 vials were made out with 12 males and 5 females in each. Two vials of 10 males each will get the control poke, and 3 vials of 10 males each will get the DiNV poke.
| Vial | Species | Treatment | Treatment_abreave | sex | Replicate | Day_emerged | Day_Infected | time_infected | fly_age | Original_N_number |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | D. innubila | concentrated cell culture medium | CCM | male | 1 | 3/22-3/23 | 20230328 | 3:53 | 5-6 days | 10 |
| 2 | D. innubila | concentrated cell culture medium | CCM | male | 2 | 3/22-3/23 | 20230328 | 3:59 | 5-6 days | 10 |
| 3 | D. innubila | concentrated DiNV #20 | DiNV | male | 1 | 3/22-3/23 | 20230328 | 4:15 | 5-6 days | 10 |
| 4 | D. innubila | concentrated DiNV #20 | DiNV | male | 2 | 3/22-3/23 | 20230328 | 4:22 | 5-6 days | 10 |
| 5 | D. innubila | concentrated DiNV #20 | DiNV | male | 3 | 3/22-3/23 | 20230328 | 4:29 | 5-6 days | 10 |
Additionally, 3 flies were poked with control medium and 3 flies were poked with DiNV fluid and frozen as a day 0 sample.
Fly mortality was measured every day for 2 weeks, and that data can be found here
Every 3 days, flies were transferred to new food and any dead flies were frozen. At the end of the 2 weeks, all flies in the experiment were frozen. Information on frozen flies can be found here