Inventory of Cell Culture and Fluid Changes, Fluid Transfers, and Passing Cells
20230228 Inventory
02/16 from ovaries
- 2 flasks
- Lots of debris in these flasks, more in one than the other
- The pieces of the ovaries look a little intact
- Maybe it is too soon to tell with these
- Want to fluid exchange these
02/23 from ovaries
- 2 flasks
- Lots of debris in the flask that had the flow through from the sieve, the other has less debris
- The pieces of the ovaries look a little intact again
- Maybe it is too soon to tell with these
- Want to fluid exchange these
02/01 and 01/31
- 2 flasks each
- Some clumps of cells have settled down on the plastic
- Not much has grown yet, might be to early to expect much
- Want to fluid exchange these
02/09 from 05/02
- 2 flasks
- Precipitate is in these flasks
- But some clumps of cells have settled, but no growth from them yet
- Want to fluid exchange these
02/09 from 10/4
- 2 flasks
- Not sure what is going on in these flasks, there do seem to be a lot of cells, but they don’t seem to be like growing out from each other, they are spaced somewhat apart
- Want to fluid exchange these
10/04
- 1 flask
- Was slightly scraped on 02/09
- It still looks like there are tons of cells in this flask, but I’m not sure if they are dead cells/debris or growing
- Want to try scraping up this flask and putting it into 2 new ones
02/09 from 12/14 and 12/13
- 3 flasks
- Doesn’t look like there are any alive cells, throw out
09/27
- 1 flask
- Lots of alive looking clumps of cells, but many are floating
- One area has a good spot of growing out cells
- Wanting to try to fluid change this flask, but put the fluid into something like a 12 well plate where maybe the clumps will settle better?
10/04
- 1 flask
- A couple of areas of good, growing out cells, but not enough yet to try to make a second passage
- Want to fluid change this flask
12/13
- 1 flask
- A couple of areas of settled growing cells
- Lots of clumps that are floating
- Want to try putting fluid from this flask into a 12 well plate
08/18
- 1 flask
- Basically seems like nothing is alive in this flask
- Thrown out
12/14
- 1 flask
- Lots of clumps of cells that aren’t settled
- Want to try putting fluid from this flask into a 12 well plate
08/11
- 2 flasks
- All cells seem to be dead
- Thrown out
09/20
- 2 flasks
- 1 flask has a nice area of growing out cells
- Want to fluid change that flask
- The other flask doesn’t have anything growing or alive looking, this flask was thrown out
01/18 from 09/13
- 2 flasks
- 1 flask has an area of growing out cells
- Want to fluid change that flask
- The other flask doesn’t have anything alive in it, thrown out
- The original 09/13 flask doesn’t really look like anything is alive in it anymore but I’ll keep it for a bit longer and fluid change it
01/12
- 2 flasks
- 1 flask looks really great with lots of areas of growing stringy cells
- This flask I want to scrape and make a second passage
- The other flask does not have that much growth but seems ok
- This flask with just get a fluid change
01/13
- 2 flasks
- Basically it doesn’t seem like any cells are alive
- Thrown out
02/09
- 1 flask
- Cells seem alive, but they just haven’t settled to the bottom of the flask
- Want to try putting fluid from this flask into a 12 well plate
09/27
- 1 flask
- Some nice areas of growing out cells
- A lot of floating cells too
- Want to try putting fluid from this flask into a 12 well plate
01/14 and 01/25
- 2 flasks each
- Not a lot of clumps of cells, but it might be to early to tell on these
- Want to fluid change these flasks
20230308 Fluid Changes and Re-flasking
All flasks were re-plenished with 10% FBS Schenider’s medium with 4% mushroom extract
Flasks to fluid change only
- For all flasks, 2mL was removed, and 2mL was added
- 02/01 2 flasks
- 01/31 2 flasks
- 02/09 from 05/02 2 flasks
- 02/09 from 10/04 2 flasks
- 10/04 1 flask
- 09/20 1 flask
- 01/18 from 9/13 1 flask
- 01/12 1 flask
- 01/24 2 flasks
- 01/25 2 flasks
Flasks to fluid change and put the fluid in a 12 well plate
- 1.5mL of the flask fluid was put into the intereior wells of a 12 well plate
- Then .5mL of fresh fluid was added to that well
- 2mL of new fluid was added back into the old flask
- 09/27 1 flask
- 12/13 1 flask
- 12/14 1 flask
- 02/09 1 flask
- 09/27 1 flask
Flasks to cell scrape and put into another flask
- 10/04
- This flask was lightly scraped, then 2mL of fluid was split into 2 new flasks, each with 6mL of fresh fluid
- 01/12
- This flask was lightly scraped, and 2mL of the fluid was put into 1 flask with 6mL of new fluid total
- The original flasks were given 2mL of fresh fluid and kept