Inventory of Primary Cells and Fluid Addition

All flasks kept had 2mL of 10% FBS Schneider’s medium added to them.

D. innubila Primaries

10/04 3 flasks rescued from plates

  • Mixed bag, some places where cells look alive, some not
  • Not any areas where cells are growing out
  • Maybe they suffered from being scraped up, keep anyways

10/05 3 flasks rescued from plates

  • Some clumps are alive but aren’t settling to the flask surface
  • No growth out but pretty much all cells look alive so worth keeping

12/14 2 flasks

  • Not a lot of cells
  • Some good clumps of cells that look alive and have settled, but no growth out
  • Also a lot of dead looking cells
  • Still fluid addition

12/13 2 flasks

  • I found 1 clump of cells contracting!
  • Many clumps of alive cells, but no growth out yet
  • Fluid addition

8/11 2 flasks

  • Not a lot of cells
  • Some cells look dead but there are some clumps that look alive, it is probably worth keeping and adding fluid

9/20 2 flasks

  • Lots of alive clumps of cells, just they are all floating
  • Fluid addition

8/21 2 flasks and 8/23 1 flask

  • Basically no alive cells - thrown out

8/18 1 flask

  • Not a lot of cells
  • Some cells look dead but there are some clumps that look alive, it is probably worth keeping and adding fluid

8/31 and 8/30 flasks

  • Very few areas of growing out cells
  • A lot of cells that look dead
  • Fluid addition anyways to keep

9/21 1 flask

  • Not a lot of good looking cells but some
  • A lot of cells that look dead
  • Fluid addition anyways to keep

05/02 2 flasks

  • Some crystals are forming in these
  • Some areas of growing cells, the flasks are just a little sparce, not a lot of cells in general
  • Fuild addition

9/27 2 flasks

  • Lots of alive clumps of cells, most are floating
  • Some areas of growing cells
  • Fluid addition

9/13 supernatant 1 flask

  • The best looking flask
  • Lots of areas of growing cells
  • Fluid addition again and think about scraping up and replating soon

IMD null

07/27 4 flasks

  • Lots of a live cells, lots of the dark clumps of stringy like cells
  • Looking good, just that it doesn’t seem to have grown any since I looked at them 2 weeks ago