Inventory of Primary Cells
D. innubila Primaries
9/13 supernatant 1 flask
- Looking good! Very large are of growth with long stringy cells
- Want to fluid change
- Think about scraping and re-plating soon
8/11 2 flasks
- Lots of large clumps of cells but they are all floating
- Not sure if these are worth keeping but I will let them sit for a while longer
8/18 1 flask
- Lots of large clumps of cells but they are all floating
- Leave to sit for longer
8/23 1 flask
- Lots of large clumps of cells but they are all floating
- Leave to sit for longer
8/24 2 flasks
- Lots of large clumps of cells but they are all floating, however not all clumps look like they are alive
- Leave to sit for longer
9/27 2 flasks
- Lots of alive clumps of cells, many of them have settled
- Little is growing out put this is still promising
- Fluid change both flasks
9/21 1 flask
- Lots of large clumps of cells but they are all floating
- Leave to sit for longer
9/20 2 flasks
- Lots of alive clumps of cells, many of them have settled
- Little is growing out put this is still promising
- Fluid change both flasks
8/30 1 flask
- Lots of large clumps of cells but they are all floating
- Leave to sit for longer
8/31 1 flask
- Lots of large clumps of cells but they are all floating
- Leave to sit for longer
5/02 2 flasks
- Some areas of good grow out, but flasks are old and growth is slow
- Still want to keep these though
- Fluid change these flasks
10/04 plate
- Plates were not a good idea, they do not stay sterile long term. The A and C rows became contaminated
- This is sad because some of the cells here look good
- I was able to save (hopefully) the B row wells: I scraped them up and put them in individual flasks (3 flasks)
10/05 plate
- Same as with the other plate, the A and C rows got contaminated
- I scraped up the B row wells and put them in individual flasks (3)
IMD null
7/29 4 flasks
- All of these flasks look pretty good, but the growth is slow
- There are many areas of clumps growing out slowly
- Interestingly there are these dark stringy cells that are growing the best, I have not seen these before
- Want to fluid change these flasks
