Trying P. rettgeri Infections Again on W1118 Flies And Poking Harder
There has been some indication that I am not poking hard enough during infections. I am trying this infection again and making sure to poke a little harder into the fly each time.
Infections followed this general protocol, and the bacteria was grown overnight in liquid cultures, and diluted to an OD of 1 before using. I had two age sets of flies, either ones that had emerged 10/19-10/22 or 10/22-10/24. I spread them out over the treatments.
Infections followed:
| Vial | Treatment | Replicate | Day emerged | Day Infected | time infected | fly age | Original number flies per vial |
|---|---|---|---|---|---|---|---|
| 1 | LB | 1 | 10/19-10/22 | 20221027 | 5:11 | 5-8 | 10 |
| 2 | OD 1 | 1 | 10/19-10/22 | 20221027 | 5:17 | 5-8 | 9 |
| 3 | OD 1 | 2 | 10/22-10/24 | 20221027 | 5:24 | 3-5 | 10 |
| 4 | LB | 2 | 10/19-10/22 | 20221027 | 5:30 | 5-8 | 10 |
| 5 | OD 1 | 3 | 10/19-10/22 | 20221027 | 5:40 | 5-8 | 10 |
| 6 | LB | 3 | 10/22-10/24 | 20221027 | 5:46 | 3-5 | 10 |
| 7 | OD 1 | 4 | 10/19-10/22 | 20221027 | 5:52 | 5-8 | 10 |
Daily mortality counts can be found here