Doing Final CO1 and p47 PCRs on DNA from Single Fly DNA Extractions and extractions to Test for DiNV Infection

Find sample number information here and PCR results from all extractions here

Samples:

  • 80
  • 20
  • 85
  • 60
  • 19
  • 73
  • 23
  • 95
  • 84
  • 45
  • 105
  • 109
  • 6
  • 24
  • 43
  • 116
  • 12
  • 82
  • 78
  • 72
  • 69
  • 5
  • 46
  • 14

PCR followed general PCR protocol using the CO1 and p47 primers, and n number of 30, and cycling conditions indicated here. A positive and negative control were used. A different positive control were used for each primer as to save my positive for p47.

A 1% gel was run for 40 min at 90V. Note that an end ladder was not added by mistake.