Redoing CO1 and p47 PCRs on DNA from Single Fly DNA Extractions and extractions to Test for DiNV Infection

There had been some suspected contamination of the PCR plates on the previous PCR attempt, so these were redone.

Samples for PCR in the layout they were in for the plates:

  1 2 3 4 5 6 7 8 9 10 11 12
A 119 92 11 28 75 91 52 120 36 31 55  
B 56 64 100 13 76 74 115 48 32 108 NEG  
C 30 3 77 8 2 53 41 7 110 44 POS  
D 40 102 68 39 54 103 104 101 33 21    
E 63 98 57 37 25 22 1 4 107 49    
F 59 106 79 18 15 66 86 29 118 26    
G 35 117 9 89 17 111 58 94 16 67    
H 38 81 34 65 113 27 70 71 96 88    

PCR followed general PCR protocol using the CO1 and p47 primers, and n number of 90, and cycling conditions indicated here. A positive and negative control were included. The plates were not vortexed and samples were pipette mixed with a multichannel.

Gels were run separately for each primer. Orientation of samples were a little weird because of multichannel limitations, however the annotations of the pictures are accurate. 1% gel, 90V, 35 minutes.

Find sample number information here and PCR results from all extractions here

CO1 gel results:

p47 gel results: