Trying P. rettgeri Infections Again on Multiple Lines of Melanogaster Flies
Lines are: W1118, c564, Dpt null 1I, and IMD null. W1118 and c564 are WT and Dpt null and IMD null are lines that should be very sensitive to P. rettgeri.
Infections followed this general protocol, and the bacteria was grown overnight in liquid cultures, and diluted to an OD of 1 before using. Lines all had flies of slightly different ages, some too young and some too old. But they were all sort of the right age, and I wanted to just see what would happen here. A few days before all flies were separated out into vials of ~10 males with ~5 females to make infection quicker day of.
| Vial | line | Treatment | time infected | fly age | Number of flies per vial |
|---|---|---|---|---|---|
| 1 | W1118 | P. rettgeri | 3:58 PM | 8-10 | 10 |
| 2 | dpt null | LB | 4:06 PM | 4-6 | 10 |
| 3 | IMD null | P. rettgeri | 4:12 PM | 3-4 | 7 |
| 4 | W1118 | LB | 4:17 PM | 8-10 | 10 |
| 5 | W1118 | P. rettgeri | 4:25 PM | 8-10 | 10 |
| 6 | c564 | LB | 4:30 PM | 4-8 | 10 |
| 7 | dpt null | P. rettgeri | 4:36 PM | 4-6 | 10 |
| 8 | IMD null | P. rettgeri | 4:42 PM | 3-4 | 10 |
| 9 | dpt null | P. rettgeri | 4:48 PM | 4-6 | 10 |
| 10 | c564 | P. rettgeri | 4:53 PM | 4-8 | 10 |
| 11 | c564 | P. rettgeri | 4:58 PM | 4-8 | 10 |
| 12 | W1118 | P. rettgeri | 5:03 PM | 8-10 | 10 |
| 13 | IMD null | LB | 5:08 PM | 3-4 | 8 |
Daily mortality results can be found here