Doing CO1 and p47 PCRs on DNA from Single Fly DNA Extractions to Test for DiNV Infection
Samples for PCR:
tube_number |
---|
99 |
50 |
62 |
90 |
10 |
61 |
87 |
47 |
93 |
112 |
51 |
114 |
97 |
42 |
83 |
PCR followed general PCR protocol using the CO1 and p47 primers, and n number of 17, a positive and negative control for each primer set, and cycling conditions indicated here
1 gel was run for both primer sets, 1% gel, 90V, 30 minutes: