Mini Cell Culture Inventory 20220815

Myd88 passage 8 from 8/11 3 flasks

  • Looks really good already
  • Could be passaged but I probably want to wait a few more days

IMD null 7/29 4 flasks

  • In every flask there are many clumps of cells contracting!
  • Most clumps of cells have settle to the bottom of the flasks
  • Lots of debris in the flasks
  • No areas that have a large amount of growth but this looks promising for the new method of homogenizing the eggs
  • The egg yield was very high for these flasks

10236 Domeless 08/11 2 flasks

  • Not seeing any contracting clumps
  • Clumps of cells have settled, they are just sparce and the cells look really small
  • There were not many eggs collected this day

10263 Domeless 08/12 3 flasks

  • More cell clumps than the 8/11 flasks
  • Not seeing any clumps contracting though
  • Some cell clumps have settled on the flasks, it might be too early to tell with these flasks

innubila 8/11 2 flasks

  • Found 1 clump contracting
  • These flasks are very sparce, some clumps of cells have settled down but they are spread out
  • Not many eggs were collected

innubila 08/12 2 flasks

  • Found 1 clump contracting
  • Very similar to the 8/11 flasks
  • It seems like there weren’t enough eggs for these flasks to get homogenized properly, I remember struggling to get all the eggs to break up

innubila passage 2 06/06 from 02/24

  • Lots of preciptate
  • Clumps of cells look alive but there seems to be little growth
  • Not many clumps of cells

innubila passage 2 06/06 from 02/25

  • Also lots of precipitate
  • More clumps of cells than the ones from 02/24
  • Some areas of cells growing out

innubila passage 2 04/25 from 02/25

  • 1 flask it ok, and has some good areas of growth
  • Another flask has some areas of growth but not as many
  • The 3rd flask had nothing alive/growing and was thrown out

innubila passage 2 04/25 from 02/24

  • Not very much going on in these flasks
  • Seems like a lot of cells are floating
  • Keep for not but little hope