Cell Culture Fluid Changes and Primary Cell Culture Weeks of 20220516 and 20220523
A fluid change was performed on all flasks in the incubator on 20220516
- 3mL fluid removed
- 3mL new 20% FBS added
1 Myd88 secondary flask from 04/22 that was basically confluent was cell scraped, and separated out into 4 new flasks on 20220516
Primary cell culture on 20220523 and 20220524
- For these cells, the egg laying scenario was slightly different:
- Flies were put on plates in mid afternoon Saturday and left overnight
- Then flies were swapped to a new plate in the morning on Sunday and left for 24 hours
- Plates were not collected until at least 10:30am to facilitate morning laying
- Then plates were left to “mature” (eggs to cellularize) for 2-3 hours after being taken off before the primary cell culture process began
- The second cc plate was put on regularly
- On 05/23 1 primary cc flask was make for IMD null flies, and 1 for innubila
- On 05/10 3 primary cc flasks were make for IMD null flies, and 2 for innubila
- These were done with the updated cell culture protocol