Cell Culture Care and Primary Cell Culture Generation for Weeks of 20220418 and 20220425

20220422

  • Fluid changed (3mL medium removed, 3mL new 20% FBS Schneider’s medium added):
    • 04/04 innubila (1 flask)
    • 04/05 innubila (2 flasks)
    • Secondary flasks from 03/30 Myd88 (6 flasks)
    • 03/09 domeless (1 flask)
  • Made 1 secondary flask from the 03/03 SPZ flask
    • Scrapped off flask surface
    • Pipetted liquid out of flask into 10mL tube
    • Centrifuged tube 5 minutes 200rpm
    • Aspirated off top liquid
    • Resuspended the pellet in 5mL 20% FBS Schneider’s medium
    • Transferred liquid to a new culture flask
  • Made 4 secondary flasks from the remaining 02/03 Myd88 primary flask. Followed the same steps as above, just mixed pellet with 20mL medium and split into 4 new flasks

20220425

  • Made primary cells from innubila following the updated protocol
    • Instead of separating out the homogenate, I centrifuged the homogenate for 2 minutes at 200rpm and removed the supernatant. Then resuspended the pellet in 5mL medium and separated into 2 flasks
  • Made 2 flasks of primary cells from 32064 dicer 2 flies, following the same as above
  • Made 3 secondary flasks each from 1 flask of 02/24 and 1 flask of 02/25 innubila primary cells. Followed the above steps from 20220422

20220426

  • Made 3 primary cell flasks from innubila the updated protocol
  • Made 3 flasks of primary cells from 32064 dicer 2 flies, following the same as above