Cell Culture Inventory on 20220315 of Melanogaster Cells

Domeless 03/09
Top

  • 1 flask
  • 10% FBS
  • Some cells and clumps that look a live, but not a lot of evidence of growth

Bottom

  • 2 flasks
  • 10% FBS
  • Looks similar to the top flask, maybe a few more clumps of cells that look alive. Some places look like there are cells growing out, which is a good sign
  • Also there are these small things/dots/debris that look different than anything I’ve seen before. Maybe contamination, but it’s been a week since this was made
  • 3mL fluid exchanged on 3/16

SPZ 03/02

  • 1 flask
  • Not very many alive cells, if any and no growth evident. This flask was tossed

SPZ 03/03
Top

  • 1 flask
  • 10% FBS
  • A lot of debris, some cells that look alive but not a lot. With the debris, probably not going to grow much?
  • 3mL fluid exchange 3/16

Bottom

  • 1 flask
  • 10% FBS
  • Could be less debris than the top flask, more cell clumps, and some evidence of growth/cell division. There was a clump contracting still

Myd88 03/02 fluid from 02/03

  • 3 flasks
  • 20% FBS
  • A lot of big dark cells that aren’t attached, some small thin cells that also look to be growing. These may be promising?

Myd88 02/02

  • 2 flasks
  • 20% FBS
  • Huge amount of debris in these flasks, no cells growing. Tossed these flasks

Myd88 02/03

  • 3 flasks
  • 20% FBS
  • Fluid changed at 3rd time on 03/16
  • Still looking really good, cells are still alive and in big grow-out groups. Hard to tell how much more growth than the last time I checked, but they still look happy
  • 10X image:
  • 20X image (same place as above):
  • 10X image:
  • 20X image (different place):

33098 dicer2 02/10 and 2/11

  • 2 flasks
  • No growth from any present cells, lots of debris. Tossed these flasks

IMD null 02/23

  • 2 flasks
  • 10% FBS
  • Cells are still alive looking, but there is a lot of debris. Not that much evidence of growth, but going to keep them anyways

IMD null 02/25. 01/26 and 01/27

  • 5 flasks including one that had fluid transferred form it
  • All had significant amounts of debris and very little cells, if any. All flasks were thrown out

Domeless 02/16

  • 2 flasks
  • No growth of cells, considerable debris build up, flasks were thrown out