Week of 20220110 Cell Culture Preparation - Domeless

Cell Culture Preparation of Domeless Eggs

20220110 Making Yeast Plates

• Made fresh yeast paste
• Then that afternoon, made 2 apple juice yeast plates with 2 steaks of yeast on each plate

20220111 Setting up Domless Flies to Lay

• Set up 6 vials of Domeless flies to lay on the yeast plate at about 2pm

20220112 Day 1 Cell Culture

• Collected the apple juice plate at ~9:45am and replaced the cage with a new plate for more laying
• Followed the Prepping Cell Culture Protocol
• Used 20% FBS Schneider’s medium, and 0% FBS Schneider’s medium with NO gentamicin made the week before (20220106)
• Flies laid a good number of eggs
• Flasks were left to settle and imaged at the end of the week

20220113 Day 2 Cell Culture

• Collected the apple juice plate at ~9:45am and disposed of the flies
• Followed the Prepping Cell Culture Protocol
• Used the 0% FBS and 20% FBS Schneider’s medium made the week before
• There were many more eggs on the plate this day
• Flasks were imaged on the 14th

20220114 Imaging Flasks

• Flasks from 20220112:
• Flasks from 20220113:

20220121 Imaging Flasks

• Cells do not look to happy/look kind of shrunk after 1 week