Watching Kent Pass (transfer) DV1 Cells to New Flasks

Steps

  • In tissue culture hood
  • Splitting 1 flask into 4 new flasks (25cm2 flasks)
  • Made two new conicals of media
    • 45mL Schnieder’s drosophila medium
    • 5mL FBS
  • Doing a 1:10 split
  • Into 1 new flask:
    • 36mL medium
    • 4mL cells _before pipetting: hard tap on the flask to break up the cells stuck to the bottom for a while. Not all cells with come off but most will. Pipette a few times to break up clumps before transferring
  • Pipette mis in the 40mL flask to mix up cells with medium
  • Transfer 10mL from the 40mL flask to each of the other 3 flasks
    • Line up flasks close and unscrew lids for ease
  • New cells are passage 17 because the flask we took from was p16
  • Placed these flasks in the new incubator (not refrigerated so it won’t go to 23, closer to 27 degrees)